Molecular identification of streptomycetes by exploiting RNA polymerase beta-subunit (rpoB) gene in Saudi arabia

This study was designed with the aim of exploring efficacy RNA polymerase beta subunit, rpoB gene analysis for identification streptomycetes. A characteristic marine environment distinguished by their content streptomycetes were targeted, and two organisms genus Streptomyces isolated in Saudi Arabia. The spp., named isolates EH1 EH2 subjected to regular phenotypic characterization including morphological physiological studies, analyses cells hydrolysates, detecting antibacterial activities pocks formation ability. These showed inhibitory activity gram-positive bacteria Staphylococcus aureus Bacillus subtilis. gave positive results test, which indicated that both must belong Streptomyces. Molecular accomplished through beta-subunit (rpoB) assist species identification. target fragments 352 bp amplified detected using agarose gel electrophoresis, indicating they product isolate sequenced 296 continuous sequence determined (GenBank accession MK569762.1). By matching obtained DNA databases, found very similar labedae. concluded possibility molecular subunit correctly identify level.